FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_76.35200000039998.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_76.35200000039998.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429000
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7240.16876456876456875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC300.001226134124.9970863
ACACAGC400.006508376418.7478146
CTGTCCA550.002100390116.3617299
TAGTAGG550.002100390116.3617292
CGCAGAT600.003748812215.018
CTAATAC1056.737957E-512.8556443
CCTTATA954.1820298E-412.6301062
ACAGGCA850.002609940512.35150058
TATACTG1702.8119757E-812.35150055
TTACAGA1006.440766E-411.9986014
GTATTAG1059.698577E-411.4272391
GTGTAGA1059.698577E-411.4272391
TACAGGC1100.001430901910.907827
TATTAGG1551.9485495E-510.643922
TTCTAAC1000.0087696110.49877552
CAGGCAA1000.0087696110.49877559
CCAGTTC1000.0087696110.49877558
ATAGAAC1150.002072210.4335673
CTTTATA1304.90614E-410.3834052
CTATAAT2053.75132E-710.2427091
Produced by FastQC (version 0.11.2)