FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_75.35200000039955.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_75.35200000039955.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073429
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41850.13616712798636313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCA1303.8505714E-511.5378169
TAGAACC5150.011.3576654
AGACTGT4150.010.1192586
GTATAGG5150.09.9021981
GTCTTAG2752.3756002E-99.8174731
TATACTG10000.09.5986825
ACTGTGG5050.09.5038018
ACTGCTC3401.10958354E-109.2635858
ATAGAAC6850.09.1956693
CTGTGCC1851.3622799E-48.9184219
TAGACGG1350.0078134468.8876695
GTCTAGG2054.3094817E-58.7798551
GTATTAG6500.08.7685991
CCTATAC4450.08.76273
TTATACT12500.08.7587984
TATACAG10300.08.7366655
ACTGTCC3101.857552E-88.7086248
TATTATA17250.08.6941772
TCAGGGC1901.8215693E-48.6828773
CAGAGGT4054.5474735E-118.5173494
Produced by FastQC (version 0.11.2)