FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_74.35200000039911.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_74.35200000039911.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440099
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21930.15228119733434994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA3300.012.2720461
TATACCG2002.2302629E-811.2485935
ACTATGC1355.6567173E-511.1097238
CCGTCGA950.006000333511.0520179
CCTATAC2053.209061E-810.9742383
AGACCGT1253.4264318E-410.798656
GTTCTAG1704.6810146E-610.5876471
ACTGTCC1000.008787028510.4986888
GTACATA2301.5503247E-810.4342031
CAGGGCA1356.9654034E-49.9987514
CTCATAC1501.645707E-49.998753
TATACAG4450.09.7740585
CTCTATA3358.185452E-119.401812
GTACAGT2403.2092794E-79.3744781
GGCTATA1603.1478843E-49.3744781
TATACTG3851.8189894E-129.3494815
TAAGACG1450.00134020229.3091814
TAGTACT3552.7284841E-119.2946134
GCCTTAT2105.66577E-69.2851981
TACCGTC2105.670772E-69.2845547
Produced by FastQC (version 0.11.2)