FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_73.352000000398dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_73.352000000398dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710418
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16990.23915497636602676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA1155.456968E-1219.5642071
TATTAGT550.002102293816.3616392
GGCTTAT600.003753329914.9992261
ATCTTAG600.003753329914.9992261
TCTAGGG600.00375508814.9981712
GTAATAT750.001017670413.9992771
TGTTATG750.001018212113.9982932
GTTAGCC650.006376138413.84836327
CCGTGTA650.00638506513.845449
CTCGGTT650.00638506513.8454412
GTGTAAG1004.2680804E-513.4993031
CATAGAC1151.0929221E-513.0418873
TCCCTAG850.002611577512.35230351
ACCGTTC850.002611577512.35230358
CCCTGTA850.002612956812.3514342
ACTGTGC1101.04231876E-412.2720948
GTATATA1501.0377717E-611.9993811
TAGACTA1006.450236E-411.9985365
ACCGCTC1151.5775305E-411.7385248
GTGTATA900.00400605311.6660641
Produced by FastQC (version 0.11.2)