FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_72.3520000003988c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_72.3520000003988c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length36
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66.382978723404255No Hit
CCGCGAGAGCGATAGAGGAGAAGTAAGTTCTGGGGA11.0638297872340425No Hit
CAACTGAGCTACGCATATATCAAAAAAAGCTCACAA11.0638297872340425No Hit
GGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0638297872340425No Hit
GTACAACATCAAGTGGAAATGTCGGAAACAGAACAA11.0638297872340425No Hit
AAGCTTACTCTAACTAACTTATAATTCAAAAGTACT11.0638297872340425No Hit
CGCAAATACTTGGCAATGAGGCTTTAGTTGAAATCA11.0638297872340425No Hit
CAATAACCTGCACAAAGTATCATTTGCTAATGCCTT11.0638297872340425No Hit
GGTGTTAGCTGGGCTTCTGATAGGTAGAGTGGATAG11.0638297872340425No Hit
CTCAAGTACTGCCGAAAAGTTGCGTATACAACCAGA11.0638297872340425No Hit
TAGTAAAACAAGTTTGATTATTTTACGTTGAGGTAA11.0638297872340425No Hit
TAAATACTCTTGAAACCATAATACAAAATCATCCTC11.0638297872340425No Hit
GAAACTCAAAGTACTCGAAAAACGTAATGTCAAAGA11.0638297872340425No Hit
ACTCTCAACAAAAGCCGCGACCACAGAAGAAACAAT11.0638297872340425No Hit
ATTCTTGATCGATCTTCAGAGTCCGACTCTGAATTC11.0638297872340425No Hit
ATATATGAACCTTTATATAACTGAAATCGAACGCCA11.0638297872340425No Hit
ATTCTATATCCAGCTCTCCATCTGTGAACCATGGAC11.0638297872340425No Hit
ATTCCACTGTATAAGATAAATACAATTTTCCCCATA11.0638297872340425No Hit
GGCACTGACCGCGCAGATGCTGCAGGCCCTGCGCAG11.0638297872340425No Hit
CAGTAAAAAGGGCACCCGTTTTTAGTGTATGGAAAA11.0638297872340425No Hit
GTTGTAGGCTAAGCGCAGGCTAAGCGTAGGTCCATT11.0638297872340425No Hit
AATAGGTCAATGCAGTCCTAAAACCCATAAAGAATC11.0638297872340425No Hit
ACATTAAATCTGTCAAAGAAGGGATAAGGGCAAGGT11.0638297872340425No Hit
ACGCAGATATCTTTTAGGGTATAATTATTATTATGT11.0638297872340425No Hit
TCTTATACACATCTGACGCTGCAGACGATCTTACGC11.0638297872340425No Hit
CGGGGGGTCGGGGGGTGGGGCGGGGGGGGGGGGGGG11.0638297872340425No Hit
GCGAGGGAGTGCGCAGCGAGGGAGTGCGCAGCGACG11.0638297872340425No Hit
CTGTTAGACGCTCCGGGGGGGAGGGGAGGGGGGGAG11.0638297872340425No Hit
TATCAAGAGTGTTACCTAGTTTGTAGCTCAAATTGC11.0638297872340425No Hit
TCGTAAAGGTGATTCAAAAGTTGATTAAATCATTAA11.0638297872340425No Hit
GAATGGAAATAGCTTGAAAAATATGGCAATTACTAA11.0638297872340425No Hit
GGGGTGGGGGGGGGGCGGTGGGGGGGGGGGGGGTGG11.0638297872340425No Hit
GGGCAGAGTGGTGGCGTTTGAGGGGCGCGATGCAGT11.0638297872340425No Hit
ATGATATGGGGTGTACGAGATCGTATAGTAGATGGA11.0638297872340425No Hit
TTATCTTGCTTTAACTTTAAGGCAAATTTACCATCT11.0638297872340425No Hit
ATACAAGAGAACTGCATAAAAGTTATAAACCAATTC11.0638297872340425No Hit
ATGGGGGGGGGGCGGGGGTGGGGGATGGGGTTAGGG11.0638297872340425No Hit
GAGAGTTAGGGTCCTGGATAACTCGAGACTGATACT11.0638297872340425No Hit
TTATAAAAATAATAATTAATAAATATTTTATAATTT11.0638297872340425No Hit
CCGTGGTTCGGTATGATGTGGGTTTTCAGCTTGACC11.0638297872340425No Hit
CATAAGCCAGTAACATAGCTAATCAACATAAACATA11.0638297872340425No Hit
TTACCAGGCTGAGACAGGCCGATCGGGCAGGGGTAG11.0638297872340425No Hit
GGGGTGAGTAGGGCGGGGGGGGGAGGGGGAGGGGGG11.0638297872340425No Hit
GGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGCGGG11.0638297872340425No Hit
CACAACGTTTATCCAATAGCCATAAGAAAAAAAGGC11.0638297872340425No Hit
GGTCGTGGTGTGGGGGGGTGGGAGGTGGCGGGGGGG11.0638297872340425No Hit
GAATAAACTGCACGAACCGACTTGAGATTCTTTTGA11.0638297872340425No Hit
ACATTATTCTCTGTCTCCAAAAATTATGGAAAATCA11.0638297872340425No Hit
AGAGGAGCATGTGTGGGGCGGGCTGAGTGGTGGGGA11.0638297872340425No Hit
AAAAATACCAGAAAAACATAATAGAAGCAATAACAG11.0638297872340425No Hit
GTAATAGAGAACTGAGGATGGGGAAGAAGAGTGAGG11.0638297872340425No Hit
AGAAGGATCAGAATAGGAGTCAAGGAGCTGCGCAGA11.0638297872340425No Hit
CCCCAGAACCGTGCTTTACCCGCTTGCCACAAACAA11.0638297872340425No Hit
CGGCCATACTCCCCCCAGAACCCAAAGGCTTTGATT11.0638297872340425No Hit
ATGATGACGTGTACACATTATCAAACGTCACCTGCG11.0638297872340425No Hit
ACATAAAATTGCTGACGTAAAAGCTTTCGAATCAAC11.0638297872340425No Hit
CTTTACACATCTGACGCTGCCGACGATCTTACGCGT11.0638297872340425No Hit
GAAATCGGCTCGGGTCGATTGATAGCAACCTAACAC11.0638297872340425No Hit
GTTAAAACACAAGATAAGCCATTCATTGACTTCTAA11.0638297872340425No Hit
GGGGGGGGGGCGGGGGGGGGGGGGGCTGTGGGTGTG11.0638297872340425No Hit
CTCGAATAGTGAGCAATTGCGCGTTATATAAAGGGG11.0638297872340425No Hit
TTAGATGACAGCCGTACTCAACTGCAATGTAGTGGT11.0638297872340425No Hit
TTGCAGAACTTGGATTTGTCGTAGTATTGGTGTAGC11.0638297872340425No Hit
AAGTCGGTCACCCCGAAAAGCGTATAGCACGCGAAC11.0638297872340425No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0638297872340425No Hit
CACCGAAATTATCACAAGGTACCTCATATACTCTCT11.0638297872340425No Hit
ATTACAAGTTGGCCACCGGCGTCGGGTTGTGTAGGT11.0638297872340425No Hit
TGCTGGTACAGTTGTTCTGGAAGCTAGATGTTGTCG11.0638297872340425No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGG11.0638297872340425No Hit
TCTATGGGTGATTACTGCCGGCATTTAGTATAGACG11.0638297872340425No Hit
GTTCACAAACGAGAATCCGGGCGTGTGGATGTCGCC11.0638297872340425No Hit
GTGGTGGGGGGGGGGGGGGGGGGGGGGGGCAGGGGG11.0638297872340425No Hit
GATTTATACAATAAATGTTTTAAACAGGATCATAGA11.0638297872340425No Hit
GTGTTGAAAATATTGCAGGAATTGGCACAAAGATTT11.0638297872340425No Hit
GGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0638297872340425No Hit
CTTTAAAAATATCATTCAATCACACTAAGAAACTTT11.0638297872340425No Hit
CGTTGGGCACGGGCGGAGAGAACGGTCTACAGCCAC11.0638297872340425No Hit
CAAACACACAGACAATTACTCAGGCATCAAGAACAA11.0638297872340425No Hit
TACTTAGAGCTTCACAATTAATGATAGCTTCGAAAT11.0638297872340425No Hit
GGGGGGGGCGGGGGGGGGGGGGGGTGGGGGGAGGGG11.0638297872340425No Hit
CTCGTTAATCCATACATGCGCGTAACAAATGAGCTG11.0638297872340425No Hit
GGTGGGGTGGGTGGGGGCGGGGGGCGTGGGGGGGGG11.0638297872340425No Hit
TACCAGCGCAAGCTCTTGACCTATTCATACAGAATA11.0638297872340425No Hit
ATACTAAAGAGTTTTAATGTTAATATATACAAGAAA11.0638297872340425No Hit
GATGGAAAAGCTTGTATTTTCGACACTTAGAGGATA11.0638297872340425No Hit
GACATAGATCATGGGACTAAGAGGACTGAACTAGGG11.0638297872340425No Hit
GAACCCGCCTGTGGTGTACTGAGAAAAAAAAAAAAT11.0638297872340425No Hit
ACCATAAACACCTCAATGTTGGGGAGTCTGTCCTCG11.0638297872340425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG11.0638297872340425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG50.029.9999988
GGGAGGG50.029.9999985
GGGGGGA50.029.9999982
GGGGGAG50.029.9999983
GGAGGGG50.029.9999986
GAGGGGG50.029.9999987
GGGGAGG50.029.9999984
Produced by FastQC (version 0.11.2)