FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_70.352000000397f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_70.352000000397f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560642
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9350.1667730922763546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAAG359.9792385E-525.7094692
CTCACTA400.006506765718.74983422
TGTTAGA600.003755402314.997192
TAGATTG600.003755402314.997195
TCCAATA1351.850458E-814.4417382
GTCATAC851.6336863E-414.1150031
TAATACC851.6336863E-414.1150034
TCATGTA650.006388592513.843562
TTAATAC650.00639236913.8423253
TATACTG1251.775521E-613.1975275
TACCCTC800.001656900613.1225415
TATTATA1101.0428822E-412.2704282
ATTATAC1101.04375154E-412.2693343
CAACCCG900.004002972511.6665629
GTTCTAT1202.3441052E-411.2478931
GTATATA1355.646038E-511.109031
TTATAGT950.005998471311.0505614
CAGTACT950.005998471311.0505614
TTTGTAC1501.3599531E-510.9969583
ATACCTT1100.001432936410.9070476
Produced by FastQC (version 0.11.2)