FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_67.3520000003973d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_67.3520000003973d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830852
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12140.14611507223909914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGT653.4419083E-416.1496986
TACACCG1301.1290467E-814.9961485
TAAGGGC650.00639495413.8425984
CTAACCT1251.7798557E-613.196614
GCCTAAT800.001658188113.1224191
TAGACAG1151.0949721E-513.0401295
ACACCGG954.194323E-412.6283366
CCTACAC954.194323E-412.6283363
TAGACTG1201.6963877E-512.496795
GACTGTT1804.6784407E-912.496797
ACAGTGC850.002615064612.3505128
ACCATGC850.002615064612.3505128
TTAGGGG850.002616245312.3497693
AGACTGT1501.0410513E-611.9969186
TACCTGT1151.5811645E-411.7361166
TAGCCTG900.00401317711.6636715
GAGGTGT900.00401317711.6636716
ATACCGT1551.5460755E-611.60992056
AAGACAG1303.8505852E-511.5354995
CAGTGTG1059.7169343E-411.42701159
Produced by FastQC (version 0.11.2)