FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_65.352000000396b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_65.352000000396b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848921
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA1456.9006455E-712.4141731
GTATTAC2802.7284841E-1110.7146141
CGTGCTA1000.00878375910.49947110
CGACTAG1000.00878375910.49947114
ACCGTGC2156.457594E-810.4645888
GTATAGG2557.188646E-910.0003061
TCTAGGA2157.249164E-79.7672132
GTTTATA3700.09.7300281
TAGGCCC1409.7197446E-49.642374
TATATGA5000.09.5997752
GTATTAG3502.0008883E-119.428861
CCGTGCG1603.1488074E-49.3745279
TATTACG1801.0100371E-49.1664522
TCTTATG2651.3841964E-79.0563922
CATACAC4150.09.0354443
ACCGCGC1550.0024577128.7092388
GTCCTGA2454.2655847E-68.57169151
TAGACAG2803.2145545E-78.5709965
GTATACA3357.656126E-98.5077231
TAACACC2651.3413974E-68.4901384
Produced by FastQC (version 0.11.2)