FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_64.35200000039671.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_64.35200000039671.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187713
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19660.1655282042042143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC800.001657620113.1237833
TACGTCG800.001657620113.1237835
CTATCCC1151.0940623E-513.0422714
TATACTG4050.012.5914275
TCTATAG1754.2373358E-811.9988883
CCTATCC1151.5798444E-411.7380443
ACACTGC1303.845725E-511.5378786
ACGGTGT1703.761852E-711.4704918
GGACTGA1059.715317E-411.4279946
CTAATAC2701.2732926E-1111.1100823
GGCTACA950.005991542711.0539351
TAGGACT950.00600093811.0516084
ACAGAGT2104.5623892E-810.7141958
TACCCTG1704.6808436E-610.5872555
CCGCTGG1000.008769710.5012381
CTTTGCG1000.008775176510.5003549
AGTCTGC1000.00878065610.499476
TTCTACG1602.7743368E-510.3124132
GTATTAG3650.010.2751851
ACACCGT2053.7722202E-710.2433856
Produced by FastQC (version 0.11.2)