FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_63.3520000003963e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_63.3520000003963e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814557
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21050.1160062759119719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGT1806.256778E-811.66813525
AGTCCCC1059.727716E-411.426868
CCGCCCG1459.34839E-611.3782349
TAGACTG1755.488964E-711.141195
GTCTAAA3900.010.76880551
TTAGACC2251.103399E-810.665074
ACTCCGC1704.6884707E-610.586658
CCTAGCG1451.1660998E-410.34584430
TACACCG1451.1690309E-410.3432795
GTATTAA4800.010.3120931
CGCCGCG1952.1971791E-610.00181124
CACCTAG1501.6420803E-410.00125928
GTATAGA5400.09.9996051
ATAGACT2403.0089723E-89.9985034
TAGTACC2905.693437E-109.8261154
CTAATAC4150.09.7575753
ACAGTCC1552.2912398E-49.6759718
GCCCGTC1409.7210373E-49.64221111
CGCCCGT1250.00414450059.59935710
CCTGTCC1250.00414733039.5985628
Produced by FastQC (version 0.11.2)