FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_62.352000000395f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_62.352000000395f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043700
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15220.14582734502251604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAC952.6361064E-514.2097098
GTGTAGA1301.8295759E-713.8453581
GCCTCAC1004.2720763E-513.49922420
GCCTAAG902.652869E-413.3325671
CTAAGCA1701.9917934E-913.2345324
GAGGTCC850.002613110512.3522319
TGGGACC850.002613110512.3522314
GTATTAT4800.012.1867991
TAGACTA1151.5789774E-411.7384565
GTATTAG3150.011.4279151
TGTGAGG1100.001433177910.90846358
GGATTAG1100.001433177910.90846351
TCTAAGC2104.5631168E-810.713673
CTAGGCT1408.17628E-510.7136694
CAGCCGG1000.00877991310.4993969
AGAGCCG1000.00877991310.4993968
CGGTATA1000.00878303210.4988932
TAGGTAG1150.002075466810.4341825
TGAGGCC1304.9117714E-410.38501416
ATAGCCC1304.916113E-410.3840183
Produced by FastQC (version 0.11.2)