FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_61.352000000395b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_61.352000000395b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460491
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24280.1662454612866495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2000.015.74845
TAGTCAG2100.014.2842649
GTATTAG3200.014.0620341
GCTCGCG750.001018168414.00001612
CTCGCGT800.001656766713.12501513
GCCTTAG1201.6945714E-512.4995861
GTCTTAG1459.338517E-611.3789341
GGTGCTC1355.6554964E-511.10998258
CGCTTAC950.005991759211.05415826
CTAACGC1100.001434300710.9079833
CTACCCC1551.9570862E-510.644084
ACCGTGC1000.00878560310.4989348
CGCAGCT1150.002077081710.43372258
ATTAGGT3750.010.3989443
GTATATA10850.010.0918311
GTGTATA4050.09.999671
TAGACTG1952.2040367E-69.9989845
CCTGGAC1356.964031E-49.9989843
TATTAGG4550.09.8894432
GGTAGTC2905.693437E-109.8265887
Produced by FastQC (version 0.11.2)