FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_60.35200000039572.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_60.35200000039572.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658749
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8720.1323721174529297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGC1106.9042653E-613.6352948
TAATACT3400.012.7931144
ACGTAAG850.00261026812.352911
ACGGTGT1151.5775909E-411.738218
ACACAGG900.004006327611.66575056
TCTATAC1303.8407605E-511.5375563
CATATAC2751.8189894E-1211.4536473
ACCATGT1202.3429235E-411.2491188
AGACTGA950.005995500411.05176456
TAGGTAG950.005995500411.05176455
TCTAGAC950.005995500411.05176453
CCTTATA1501.3579693E-510.9991372
TCATATA3350.010.7454252
TCCTATA1551.9520981E-510.6443262
GTATAGA2402.6375346E-910.6249731
CTAATAC1851.1181528E-610.5397143
CTAGACA1000.00877550710.4991764
ACACTGT1000.00877550710.4991766
CAGGCAA1150.0020740810.4339659
TACCGTA1150.0020740810.4339657
Produced by FastQC (version 0.11.2)