FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_59.3520000003953f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_59.3520000003953f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123300
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13120.11679871806285053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAG400.006511522518.74944318
GGGCTGC400.006518220518.7461056
ACTGTCC908.6006185E-716.6632048
GTATAGG2000.014.2476741
GCTCGGA902.656528E-413.3305642
GTCTATG1507.395283E-812.9978771
CCTAGAC1056.762644E-512.8544723
CACTGTC1404.5174238E-712.8544727
CCGTCGC1056.762644E-512.8544729
CTCGGAG954.1946306E-412.6289553
TAGCCTG1201.6967891E-512.4974045
ACACTGT1804.6838977E-912.4974036
CTAGACA1601.7167986E-712.1849684
TCTATAC2600.012.1128683
GGCTCGG1006.45762E-411.9980411
GCCGCGT900.00399855111.66995725
CGCCGCG900.00399855111.66995724
AGTGTCC900.004013211511.6642448
TATACTG3700.011.3489925
GTATAAC2402.2191671E-1011.2481631
Produced by FastQC (version 0.11.2)