FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_58.352000000394fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_58.352000000394fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708971
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13260.18703162752778324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC852.2158929E-819.411087
TAGTCAG1007.2541297E-917.9993659
CGATCAC550.002099089816.36536824
TAGGTAG1052.4769724E-715.7126235
TTAGGTA1351.8511855E-814.4429164
GAGTGTG1004.267403E-513.4995247
GAGAGTC800.00165575713.1245377
CCTGCAT954.1851468E-412.6311338
TAGACAG1006.449332E-411.9987315
AGGCTGT1303.840028E-511.5380556
TATACAG4050.011.1099365
GATTAGG950.005997485511.0514631
AGTACCG1100.001432772810.9079375
GTATTAG1951.8925493E-710.7680911
CTCTTAT1408.171779E-510.7131521
TATTAGG2104.5560228E-810.7131522
ATGTTAG1408.171779E-510.7131521
GTATAGA2701.546141E-1010.5544391
CGCTGAG1000.00877380710.4996314
TTTAGGT1000.00877839610.4988893
Produced by FastQC (version 0.11.2)