FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_57.352000000394b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_57.352000000394b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507230
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA350.00300575521.4262491
TAGTCAG904.2682586E-818.3295389
TAGACTG653.437299E-416.1505035
TAGGTAG1206.862501E-814.9968945
AGTCAGA1402.9836883E-813.92568810
TTAGGTA1301.826611E-713.8432884
TATTGCG650.006388318713.8432885
GGTAGTC1201.1213269E-613.7471527
GTAGTCA1352.8930117E-713.3305748
GTATTAG1601.2070814E-813.1235771
TACTGTG1056.7483954E-512.8544817
GGATATG954.1840825E-412.6302091
CTACACC954.187564E-412.6289644
GTCATAT1456.879309E-712.4124471
AGGTAGT1702.8199793E-812.3503846
ATTAGGT1702.8199793E-812.3503843
CTAGTAC850.002612845712.3503843
ACTGTGT850.002612845712.3503848
ACTGTCC850.002612845712.3503848
GTATAGA1101.04173705E-412.2713961
Produced by FastQC (version 0.11.2)