FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_56.35200000039473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_56.35200000039473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761120
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13920.18288837502627706No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTG350.00300618621.4273999
CTATGCA1401.07320375E-1016.0705499
CTTACAC951.5092646E-615.7875723
GTATTAG2050.015.3680411
TAGTCAG1206.86523E-814.999189
GGTAGTC1104.200083E-714.9981937
TATTAGG2051.8189894E-1213.9016792
TTAGGTA1454.7348294E-813.4466564
ATTAGGT1905.4569682E-1113.4194363
CAGTCTC1352.894085E-713.3326039
TAGACAG1601.21090125E-813.123425
TGATCGA954.1864655E-412.63088710
ACAGGTC954.1864655E-412.6308878
TAGGACT954.1887863E-412.6300574
TAAGACC1201.6935723E-512.4984954
AGACAGT1456.8954614E-712.4122986
GTATTAA1702.8147042E-812.3546991
GAGAGCC850.002613222212.3514547
GTATAGA2204.7293724E-1112.2744741
GCCTTAT1006.436848E-412.0017091
Produced by FastQC (version 0.11.2)