FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_55.35200000039430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_55.35200000039430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037939
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15950.1536699170182448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT400.00651062318.7497312
CTATGCA2800.014.463389
TACACCG1351.8546416E-814.4428435
TCACACA2000.014.25116830
ACTATGC3750.013.9991238
TAGGTAG1504.9003575E-913.9984485
TAGGGTC1004.2741718E-513.4985055
ATACCGG800.001656999913.1241786
TATTAGG3200.013.1235462
ATCACAC2550.012.94223629
CGCTCCT1056.743357E-512.85819629
CTAATAC3850.012.8557193
TAGTCAG1651.8637365E-812.7264769
CCTAGCG850.002610362812.35395230
CCTGCCC850.002613194512.35216710
TAGGACC850.00261413912.3515724
TAATACT5650.012.2110364
TAAGACT1601.7141429E-712.186154
GGTAGTC2102.8921932E-1012.14209657
ACTAAGC1252.5696456E-512.00156125
Produced by FastQC (version 0.11.2)