FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_54.352000000393fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_54.352000000393fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336503
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23630.17680469104820568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC951.5115747E-615.7873468
AGCGACC650.006392467313.8442898
GTATTAG3700.012.9721971
GTAGACC1651.868284E-812.7250824
GTCTATC1456.9034286E-712.4130511
TAAGGAG1857.03767E-912.16006855
CTATACT3050.011.8012484
ACCTCGC900.004011701311.6650968
CCGCTCA1303.8498736E-511.5369079
GGTTAAC1202.3459546E-411.2493261
AGGACAG1602.266317E-611.2480655
ACCTAAG1355.6522666E-511.1104451
CCTGTGT1355.6565754E-511.1096148
CCATGCG950.00600348511.0511439
GGACAGG950.00600348511.0511436
TACACCG1653.278592E-610.9072135
CTAATAC3500.010.7124413
TATACTG4800.010.6231715
ACTGGTC1704.684627E-610.5868098
CCGTCGC1000.00878710710.4985859
Produced by FastQC (version 0.11.2)