FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_53.352000000393b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_53.352000000393b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486816
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCG8410.17275520936041544No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACG6780.13927233287320057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4920.10106487872214553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC400.006506258618.74948513
CACGGAC500.00110841318.00135625
CTGGGCT500.001109177517.9995084
TTAGGTA550.002099685816.363194
GTATAGC809.7275355E-515.0011311
GTATTAG1002.5641075E-615.001131
CTCCTAA600.003747902615.001131
ACAAGCC706.029253E-414.999598
GTGCATT600.003750465314.99958921
GCACTTA600.003750465314.99958912
GTCTTAA650.00637589513.8471981
GGTGCTC650.00638023613.8457758
GGTGCAT650.00638023613.84577520
GTATAAT1855.020411E-1012.9739491
TGCGACG1907.6579454E-1012.63253130
ATCTTGG850.002607056412.3538711
AGGGTAT850.002609066412.3526046
GTGCGAC1856.9667294E-912.16307729
TTTGAGC1806.2194886E-811.66754424
GTATAGA1303.8299364E-511.5393311
Produced by FastQC (version 0.11.2)