FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_52.35200000039367.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_52.35200000039367.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924143
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15330.16588341847527927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG957.939889E-817.3650075
AGGGGCG550.002103985516.360425
AGAGCGA901.5822057E-514.99867410
GTATTAG2400.014.373731
CCCGGAT650.00637709713.84867724
GTAGACA1151.5806466E-411.7368234
GTATAAA3850.011.6872791
TTAGGAC900.00401195511.6643733
CTTACAC1551.5455753E-611.610623
TAGGTAG1303.8493105E-511.5361925
GGATAAG1703.7622704E-711.4695751
AGCTGTC1059.6928276E-411.43065530
AGACAGC1202.34744E-411.2477886
CTAATAC3200.011.2477883
GCCTTAG950.005999040311.0516551
GTGTAGG950.005999040311.0516551
AGACCAC950.00600387511.0504595
CAGGACT1100.001434573110.9069464
ATCACAC2251.094304E-810.66861129
TAGACAG1551.957E-510.6430685
Produced by FastQC (version 0.11.2)