FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_51.35200000039324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_51.35200000039324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541209
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21550.13982529300049507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC402.4687967E-422.4989788
AGTGCGG809.7566604E-514.99980610
TCGCGAG600.003755019314.99980512
CCACGCA800.00165701513.124839
GCTGGAC800.001657824713.1239793
TTAGGCT1551.1344855E-712.5796664
TAGGTAG1754.2402462E-811.9990655
CGGCCCC900.00400853311.66651510
TGGACCG950.00600165411.051775
GTATTAG4850.010.8253041
TGTGCGA1000.00878020410.49986410
CTTATAG2752.1645974E-1010.3628293
CGCTCAG1200.00295515319.9998710
TAATACT7500.09.9992214
TCCTAGA1653.8977545E-59.9992212
CATATAC7600.09.8676523
GGTAGTC2609.888026E-99.8072477
TAGTCAG1851.28088395E-59.7296049
CCTATAC3101.8553692E-109.6766663
ACACCGC1409.7202434E-49.6421066
Produced by FastQC (version 0.11.2)