FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_50.352000000392ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_50.352000000392ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898484
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14240.15848918845522011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG400.00651534518.7470449
CTAGGGT400.00651534518.7470444
TCGGTCG400.00651534518.7470449
ACAGCGC550.002103377816.3610558
ACGGTGT1553.092282E-1115.481438
ATAGGAG1156.951177E-714.3455653
GTCTTAG851.6334606E-414.1169921
ACCGATC650.00639129113.843978
GGACCAC650.00639129113.843976
CTATCCC800.001657885213.122934
CTTAGGC800.001657885213.122933
TAGTCAG1201.695024E-512.498039
TCTAGAC850.002614585212.3509943
TAGAACT1951.1659722E-912.3057524
TTAGGCA1101.0439451E-412.2707924
ATAAGCC1101.0439451E-412.2707923
GTATTAG1857.012204E-912.1615981
TACGGTG2102.8921932E-1012.1409437
GTCCATA1754.229514E-811.9994421
TAGGTAG1252.5763266E-511.9981075
Produced by FastQC (version 0.11.2)