FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_49.352000000392b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_49.352000000392b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851049
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29540.159585186561782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC455.4743676E-419.9967043
TAGGGAG1106.9266334E-613.6341165
CCGGACG902.6533753E-413.33329712
TAGGCTG800.001659130413.1228385
TCTAGAC2102.910383E-1012.1408563
AGTCCGA1252.5778387E-511.99899510
GCCGGAC1151.5794355E-411.739099511
CCGGACA950.006006309811.0508114
TAGACAG3700.010.9441435
GTATTAG4600.010.76055051
TCTACAC3250.010.6136363
CTAGACA2556.4210326E-1010.5864914
GTATAGA4700.010.5316021
GTCCTAG1000.00878241410.4996881
TAGGGGG1000.00879121210.498275
ACGGGCG1602.7789367E-510.31163610
TATTAGG5050.010.0973452
CCTAGCG1200.002950750310.00186530
AACGCGA1200.0029573649.99916410
TACCCCT1200.00295935089.9983535
Produced by FastQC (version 0.11.2)