FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_48.35200000039275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_48.35200000039275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280479
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4240.15116996281361528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAC400.006498512318.7499981
TCGAACC400.006498512318.7499989
GACTAGG400.006498512318.7499988
GCCGCGT601.8568028E-417.5031225
CGCCGCG550.002096316316.36363624
AACACTG550.002096316316.3636365
GGTATAT600.003744502615.01
TCGCCGC600.00374894914.99732623
GTATTAT1054.242089E-614.2857131
ATTAGGT650.0063701913.8461533
AGTACCG650.0063701913.8461535
TTATATA1201.1135944E-613.752
GTATTAG954.1699084E-412.6315791
ATACTGA954.1699084E-412.6315796
TATACTG954.1699084E-412.6315795
GTATTAA850.00260377512.35294151
TATAGAA850.00260377512.35294152
GTATAGA1006.4222165E-412.01
ATATACT2052.5193003E-911.7073174
TACAGTA900.003994183611.6666662
Produced by FastQC (version 0.11.2)