FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_47.35200000039232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_47.35200000039232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553986
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6870.12401035405226847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG400.00651391418.746225
GCAGATC500.001110737917.996378
GGCGTCC550.002098822716.36476921
CGCAAAG550.002098822716.36476912
TAAGGCG653.4307854E-416.15496418
ACATGAC653.437872E-416.150593
ACTATGC1156.940718E-714.34493358
GGATACG851.6325778E-414.1160759
GTATCGG750.001018327513.9971771
GCGTCCT650.00637761613.84711322
TAAGGCA650.00638906913.8433634
TACACTG1056.750235E-512.8545515
CTATGCA954.1852662E-412.6301739
AAGGCGT850.002607960512.35379619
GTACACA850.002613264412.35045051
TCTATAC1354.2061383E-612.2197583
CTAGAAC1006.4505986E-411.9975813
CCTAATA1501.038281E-611.9975812
GTATTAG1652.551551E-711.81581
TTAGTAC1406.3115112E-611.7833393
Produced by FastQC (version 0.11.2)