FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_46.352000000391fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_46.352000000391fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378636
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6310.16665082031291267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG608.240113E-619.9963875
GTATTAG908.549414E-716.665861
ACTGATC706.0197763E-415.0012568
AGAGTGT706.0197763E-415.0012566
TACAGGC600.003745640215.0012557
GTATAGA706.0258183E-414.9992741
GTATTAA1156.9119596E-714.3471311
GATGTAT1004.257641E-513.4993471
GGTTTAT1004.257641E-513.4993471
AATACAG1454.713911E-813.44584755
TTACACA800.001655085713.1226294
AATACCG1056.737925E-512.8548215
TCCTATT954.182268E-412.6292972
TAGACCT850.002610212712.3507114
GTATATG1101.0398048E-412.2721331
ATACCGT1006.426844E-412.0010046
TGTTACA900.004003998411.6645592
TAATACA1551.5373262E-611.6108074
ATACAGT1303.8243557E-511.5394276
ATAATAC1859.1193215E-811.3478033
Produced by FastQC (version 0.11.2)