FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_45.352000000391b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_45.352000000391b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258603
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCGC350.002998466621.4303689
CCTGCAG500.001109316117.9945453
ATACAGT703.1002797E-517.1409786
TCTACTA550.00209725516.3618432
ACGGTGT600.003746177314.9983568
GTATATA1802.1827873E-1114.1651141
TTATGCA851.6299187E-414.113374
ATAGATG750.001016527613.9957593
ATATACG750.001016527613.9957594
GTATTAG650.0063730413.8446361
TATACGG650.006381213713.8419585
GTAGTAC650.006381213713.8419583
CCTAATA902.6419657E-413.3318722
ATTAGGT800.001654140813.1210243
ATTAGAT1151.0885695E-513.0395273
AAAACAG1101.039816E-412.269015
TCATATA1006.4251415E-411.9986852
CTAATAT1303.8285267E-511.53496553
TATTAGA950.005980206711.0514212
ATAATGC950.005988825611.0492833
Produced by FastQC (version 0.11.2)