FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_44.35200000039176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_44.35200000039176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412798
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5880.14244255059375288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTAG350.003002787221.42874328
CAGAGTT550.00209846116.3637684
CTATTAC600.003745270715.0019391
GTGATTA600.003748291415.0001229
AGTACTG600.003748291415.0001225
GTACTAT851.6276553E-414.11947251
TATAGAC851.629332E-414.1177623
GTACTAG750.001015012514.0018091
CAGTGTA750.001015942814.00011359
CAGCGTA650.00637656913.8462669
GTTATAA800.001651669513.1266971
TAATACT1404.4824628E-712.8572464
GTATAAT1201.6848093E-512.5016161
TCTAAAG1006.4331107E-412.0000972
TCATAAC1006.4331107E-412.0000973
TAGGGAA1303.8292746E-511.5385555
GTATAAA1602.2457298E-611.2514541
GAGTACA950.00597982511.054061
TTAGAAC950.00598522111.0527213
GTGTACA1100.001427770610.9105011
Produced by FastQC (version 0.11.2)