FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_43.35200000039126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_43.35200000039126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320050
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840.11998125292922981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGATC400.006501841418.7493158
CCTCAAC400.006501841418.7493153
CTGGAGT400.006501841418.7493154
AAAGTGT500.001108116417.9993426
CTAAAGT550.002097698616.3630394
TGGGTGC653.4278864E-416.1532576
TGTACTG653.4278864E-416.1532575
GCCTCGT600.00374695214.99945419
AGCACTA600.00374695214.9994545
CTAATAC706.0216704E-414.9994533
CTGCTCA750.001015408813.999499
GGGTGCT750.001015408813.999497
TAGACAG750.001015408813.999495
GTTTACG650.006374323313.8456491
TCTAGGA650.006374323313.8456492
ATACTCT650.006374323313.8456494
ATAAGGG650.006374323313.8456493
GGACAAT650.006374323313.8456496
CCTAATA1106.874763E-613.6358662
CCTTTAG902.643409E-413.3328471
Produced by FastQC (version 0.11.2)