FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_42.352000000390e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_42.352000000390e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862551
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14900.17274340879553787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGCC952.6362715E-514.2090174
CCGGATC851.63176E-414.11860225
TTAGGCT650.006388841713.8446834
TAGGACT1004.2723528E-513.4985654
CCAGGAC954.189289E-412.6302383
TATACTG3350.012.535985
TAGGTAG1201.6939446E-512.4986715
CACGCAG850.00261229512.352344516
TAGAGTG1354.209325E-612.2209235
ACCCGGA1006.445569E-412.00011523
CTAGTAG900.0040088811.6654263
GTATTAA2503.8198777E-1111.4007721
CTAGAGT1459.333755E-611.3781014
CGGATCA1202.341348E-411.25076126
ATACTGT2801.8189894E-1211.2488056
TACATAG1755.473139E-711.1416735
CCTTGCG1355.6428846E-511.1112189
TCTGGAC1355.649551E-511.109933
CCCGGAT950.0059915311.0533824
TTAGACA1901.3237513E-711.0514574
Produced by FastQC (version 0.11.2)