FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_41.352000000390ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_41.352000000390ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715818
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC500.001110621117.9976963
TATCCTG952.635578E-514.2087075
TCACACA902.6479282E-413.3344230
CTAGTGC800.001656941513.123323
TAGGTAG1151.0930185E-513.0418075
TATAGGG954.188513E-412.6299615
ATTAGAC1201.6933725E-512.4983993
GTATTAG2051.9281288E-1012.4382991
GTTAGAT850.002613109312.351363
GTGTAAG1101.0424042E-412.2720131
TAAGCCT1354.207528E-612.2206574
GTTCTAG1006.446885E-411.9993011
ACCGAAT1151.5786865E-411.7376278
AGAGTGT1303.843791E-511.5369846
TGACCAG1202.344544E-411.2485595
TAACCAG1355.6476943E-511.1096895
GACTGTG950.00599857611.0512167
GGATTAA1100.001432233610.9084561
AAGACTG1653.2699227E-610.9076945
TCCTATA1100.00143306910.9076942
Produced by FastQC (version 0.11.2)