FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_40.3520000003906a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_40.3520000003906a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324904
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4510.1388102331765691No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCG3320.10218402974417058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG254.2187137E-430.0012330
CCGTCGT300.00122436825.00102629
CGTGAGC350.00300085221.42945112
GACCGTC350.00300085221.42945127
AGCTGGG400.006499137718.7507716
AACGTGA500.001107589118.00073810
GCTGGGT500.001107589118.00073817
GGTGCGA500.001107589118.00073828
GTTCCAG550.002096704216.364311
TTCTAGT600.00374518315.0006151
TAATCCT600.00374902114.9983074
GTATTAG750.001014872214.0005741
GTGCGAC650.00637132713.84672229
CCAATAC650.006377828313.844593
TTATACT1004.2565873E-513.4984774
TATACTG1251.7674865E-613.1985095
ATACAGG1651.8473656E-812.7258376
TACAGGC954.1770484E-412.6301547
TCCAATA1456.8542795E-712.4123922
AGAACGG850.00260450712.35344821
Produced by FastQC (version 0.11.2)