FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_39.35200000039027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_39.35200000039027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596144
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7190.12060844359752007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCG400.006510582818.74816311
GTGTTCC706.035299E-414.9985319
ACATCTC706.0391426E-414.9972738
GACTGCT600.00375563714.9972737
TAGGTAG1106.909E-613.6338855
TTACACT1507.374183E-812.9976374
CTAGACC850.002613277212.3506974
GTATTAG1354.202777E-612.2210261
GTCTTAA1151.5773934E-411.7379821
TAGTCAG900.004006144611.66552459
GTAGAAG1303.843382E-511.5363653
TACTATG2259.094947E-1011.3312737
CCATAAC1202.3444601E-411.2479553
CTGTGCT1100.001432097410.9080239
GTATTAA2351.8644641E-910.8500021
GTATAAC1253.4192138E-410.79894351
ATTAAAC1851.1189641E-610.5386263
GTGTTAG1000.00877512710.4989721
ACAGTGC1000.008780586510.4980928
AGACCGT1000.008780586510.4980926
Produced by FastQC (version 0.11.2)