FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_38.35200000038fe5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_38.35200000038fe5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660582
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8770.1327617161836078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT653.437282E-416.1517284
TAGCCTG706.0380495E-414.9980335
CCTTATA851.6336048E-414.1157942
TATACTG851.6336048E-414.1157945
TACACCG650.00638780713.8443385
TGATGTG1201.1218435E-613.7481965
TATGACT1101.0428799E-412.2711174
GTCCAGT1006.4373773E-412.0011511
TCCAGCC900.00400792711.6651367
TTATACT1703.758123E-711.4690834
TAAGGGT1059.7119046E-411.4270724
TAAACTA1202.3440906E-411.2485245
TCAAGGA1355.6463483E-511.1096533
TTCTAGG950.005997885511.0511822
ATACTGG1100.001432852610.907666
GAACGTG1100.001432852610.907669
GTACTAA1000.00876421110.5010071
GACTGTG1000.00877898110.4986237
GTATTAG1150.002071087710.4357831
GTATTAC1150.002071087710.4357831
Produced by FastQC (version 0.11.2)