FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_37.35200000038fa2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_37.35200000038fa2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948917
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15520.16355487360854531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATT600.003755207614.9988417
ACAGAGC809.755725E-514.9988418
AGACTGT1857.017661E-912.16122156
AGACTGC1303.8448758E-511.537576
GTTCTAG1059.7100605E-411.428291
CCAAGAC2103.739842E-911.4270863
CCCTTAC1202.3438224E-411.2497241
ACTGCTC1602.2614659E-611.249138
TATTAGA3350.011.19257452
ATACTGC2155.4023985E-911.1619296
CCTTACA1755.4770317E-711.1414082
TATTACG950.006001099511.0511952
GTCTTAT1551.9534345E-510.6448981
TAGACTG1704.679714E-610.5868595
TTACCCC1000.00878364810.4986354
CTATACT2301.5488695E-810.4334274
GTATATA5650.010.3537281
CTACTAA2053.767418E-710.243651
CTTACAC2053.772875E-710.2425713
CAAGACC2209.029645E-810.2259444
Produced by FastQC (version 0.11.2)