FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_36.35200000038f6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_36.35200000038f6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486563
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6800.1397557972965474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTA805.165808E-616.8732664
CAGACAG706.032699E-414.9984585
TTGGACC954.1833372E-412.6302814
GTGTATA850.002610539312.3516721
AGCCTTT1101.0415134E-412.2714666
GTATTAC1354.1985295E-612.2209661
TGTTATA1151.5763247E-411.7379242
GTGCTCA900.00400448311.6654669
GTTATAT1059.70155E-411.4273971
TTTATAC1602.2547265E-611.2488443
ATCTGTG1100.001431336410.907977
ATAATAC1253.4169326E-410.798893
CTACAGT1551.9497349E-510.6440684
GTATAGA1551.9497349E-510.6440681
AGACAGA1000.008771555510.4989216
GTATTAA1200.00295115229.9989731
TAGCCTT1356.946365E-49.9989735
TATACAA2157.2099647E-79.76643855
TTATATA2003.0305127E-69.7489992
TTTTATA1851.2765002E-59.728732
Produced by FastQC (version 0.11.2)