FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_35.35200000038f1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_35.35200000038f1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838730
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21980.11953902965633888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG1056.761277E-512.8556745
TAATACT5800.011.1194194
TAGGACC1355.6580728E-511.1098424
GTCTATA4000.010.4996571
ACTGTGC2156.458504E-810.464498
TATTATA7350.010.2029172
GTCTTAG2957.2759576E-1110.169161
ATTAGGC1501.6465418E-49.9985873
GTATTAT5900.09.914931
GTATAGG2003.0448973E-69.7496811
ACCGGCT1705.4095304E-59.7053018
ATAATAC6400.09.6080163
GTATAAT6000.09.499691
ACCATGC3001.0531949E-99.4994328
TAGCCCT1901.7594926E-59.4726024
CTACGAC1757.436125E-59.4272383
TTAGGAC1300.0057295759.2294653
GTATAGA5550.09.18888951
CTATTAG2957.932613E-99.1522441
TAAGACA3306.1118044E-109.089874
Produced by FastQC (version 0.11.2)