FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_34.35200000038ed9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_34.35200000038ed9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777198
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13060.16803954719389397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC706.032661E-415.0003544
CCGGTCA650.00638259713.846489
CCTATAC1201.1214324E-613.749443
CTAGACA954.1838747E-412.6318784
TAGACAG954.1838747E-412.6318785
GTTTAGG850.002610485312.3532331
GTATTAG1754.2220563E-812.0002831
GTATAAT3050.011.8035571
AACTGTA1901.3206409E-711.0528937
ACAGGGT1100.001431495510.9093488
TACAGGG1100.001431495510.9093487
TAAGGGT1408.163374E-510.7145394
CTAGGAC1000.00877543710.4995733
CTTACAC1000.00877543710.4995733
CCTTTGC1150.002073042610.4350298
GTATTGG2802.9831426E-1010.1788111
GTATATA4600.010.1089341
GACAGTT1200.002951445810.00023657
GTTTTAG1501.641032E-410.0002361
TTAGACA1653.886655E-510.0002354
Produced by FastQC (version 0.11.2)