FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_33.35200000038e96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_33.35200000038e96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287269
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACA200.005142220829.9984322
CCTTATG350.003001639921.4274522
GCACTTA400.006500848618.749026
ATACCGT500.00110785817.999066
ACAGAGC601.8602808E-417.4990868
CTATTAT550.0020923416.368481
TATGGCT550.002097215716.3627824
TCTATAC1052.4605106E-715.7134663
TAGATTG1002.5586342E-614.9992165
AAATACT750.001015084613.99926954
TCTATGA750.001015084613.99926952
CCATATA650.006372892313.8454312
TATAGAC650.006372892313.8454313
TATTATA1106.8700137E-613.6356522
GTTCTAA800.001647470213.1288841
ATCTATA1151.08675995E-513.0427972
CATCTAT850.002598264312.3565971
GTCTACA850.002598264312.3565971
GTCTTGG850.002598264312.3565971
TTACACA850.002605062412.3522964
Produced by FastQC (version 0.11.2)