FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_32.35200000038e53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_32.35200000038e53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689705
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10270.14890424166853944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG400.006507281718.7503175
TCTAAGC1203.8980943E-916.2502773
TAACTAG755.6148197E-516.000275
GTATTAA2000.015.7502661
CTTTGCG706.031748E-415.0002539
GTCTTAG1002.5694317E-615.0002531
CGATCAC600.003753280714.999166524
ACCTAAT1658.185452E-1114.54571
CTGAGGC851.631715E-414.11788715
CCTTAAG650.006381917313.8463891
CGGTTAA650.006381917313.8463897
AGTGGTC650.006381917313.8463898
CTGCGCA650.006381917313.8463899
TTCCTAG650.006381917313.8463891
CTAGCAT1454.7237336E-813.4485033
GCCTCAC902.6507833E-413.33259220
TCTAGCA1601.207809E-813.1252222
GGGTATA1056.741085E-512.857361
TGAGGCC1056.741085E-512.8573616
ATACTGA2001.2732926E-1012.7502166
Produced by FastQC (version 0.11.2)