FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_31.35200000038e1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_31.35200000038e1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603172
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24000.14970321337947518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCC706.0448505E-414.9979899
GTATTAG4400.014.6603251
TAGGACT750.001019286213.9981234
TAGTCAG2500.013.7981499
TATTAGG4150.013.3721182
CTAATAC5200.013.2674513
TAGGTAG3000.012.9982575
GCCTCAC1056.75556E-512.85662120
GGTAGTC3000.012.4983237
ACTATGC4700.012.1260348
GCCGCGT1501.0379881E-612.0017625
GTCTAGA2001.7353159E-912.001011
CCTATAG1501.0415151E-611.9983913
ATCACAC3150.011.90725129
CGCCGCG1406.310129E-611.78707524
CCTAATA5400.011.6654652
ACTGGTC1951.535227E-811.5369148
CTATGCA3800.011.4458329
GAGGCCC1355.6581703E-511.1096219
TCACACA3400.011.03137430
Produced by FastQC (version 0.11.2)