FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_30.35200000038dda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_30.35200000038dda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758661
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31270.17780572833536423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2100.014.9982385
CTGTGCG1054.278063E-614.2840369
CGGCGTC650.006381970413.8480725
GGGGCTC1056.761157E-512.85563310
ACTACGC1302.7643837E-612.6908168
TGTCCGA1201.6965067E-512.49853110
TCCTAAG2307.2759576E-1212.3898482
AGCCCTA850.002615515412.3514910
GTATTAG5500.012.272681
GTATAGG3600.011.2499581
CTAATAC5900.011.1851263
GGTAGTC2207.765266E-910.9078087
CGGCATA3350.010.74775625
GTCCTAA2104.568392E-810.7142451
GGCGCCG1000.00877118210.50145325
CTGGTCG1000.00878783710.4987669
TTAGGTA3450.010.4335574
ACCCCGT1150.002077719410.43355710
TCTAACC2905.2750693E-1110.3436123
CCAGGAC1602.7797552E-510.3112883
Produced by FastQC (version 0.11.2)