##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_l04n02_ntr_3.35200000038723.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8 Sequences flagged as poor quality 0 Sequence length 36 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.75 0.0 0.0 0.0 0.0 0.0 2 32.0 0.0 0.0 0.0 0.0 0.0 3 32.0 0.0 0.0 0.0 0.0 0.0 4 29.75 0.0 0.0 0.0 0.0 0.0 5 27.5 0.0 0.0 0.0 0.0 0.0 6 33.25 0.0 0.0 0.0 0.0 0.0 7 35.5 0.0 0.0 0.0 0.0 0.0 8 32.125 0.0 0.0 0.0 0.0 0.0 9 32.125 0.0 0.0 0.0 0.0 0.0 10 35.5 0.0 0.0 0.0 0.0 0.0 11 32.75 0.0 0.0 0.0 0.0 0.0 12 33.25 0.0 0.0 0.0 0.0 0.0 13 34.125 0.0 0.0 0.0 0.0 0.0 14 33.25 0.0 0.0 0.0 0.0 0.0 15 34.5 0.0 0.0 0.0 0.0 0.0 16 33.25 0.0 0.0 0.0 0.0 0.0 17 33.625 0.0 0.0 0.0 0.0 0.0 18 32.75 0.0 0.0 0.0 0.0 0.0 19 35.5 0.0 0.0 0.0 0.0 0.0 20 34.875 0.0 0.0 0.0 0.0 0.0 21 29.5 0.0 0.0 0.0 0.0 0.0 22 35.5 0.0 0.0 0.0 0.0 0.0 23 33.25 0.0 0.0 0.0 0.0 0.0 24 32.75 0.0 0.0 0.0 0.0 0.0 25 33.25 0.0 0.0 0.0 0.0 0.0 26 35.5 0.0 0.0 0.0 0.0 0.0 27 33.875 0.0 0.0 0.0 0.0 0.0 28 35.5 0.0 0.0 0.0 0.0 0.0 29 32.75 0.0 0.0 0.0 0.0 0.0 30 32.125 0.0 0.0 0.0 0.0 0.0 31 30.0 0.0 0.0 0.0 0.0 0.0 32 30.0 0.0 0.0 0.0 0.0 0.0 33 32.125 0.0 0.0 0.0 0.0 0.0 34 33.75 0.0 0.0 0.0 0.0 0.0 35 32.75 0.0 0.0 0.0 0.0 0.0 36 35.5 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 28 1.0 29 0.0 30 2.0 31 0.0 32 1.0 33 0.0 34 1.0 35 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.5 12.5 37.5 12.5 2 25.0 25.0 37.5 12.5 3 50.0 12.5 12.5 25.0 4 25.0 25.0 37.5 12.5 5 0.0 87.5 12.5 0.0 6 12.5 62.5 25.0 0.0 7 37.5 37.5 0.0 25.0 8 50.0 25.0 12.5 12.5 9 37.5 12.5 37.5 12.5 10 12.5 37.5 50.0 0.0 11 12.5 37.5 37.5 12.5 12 25.0 25.0 37.5 12.5 13 37.5 12.5 25.0 25.0 14 25.0 37.5 25.0 12.5 15 37.5 0.0 37.5 25.0 16 25.0 50.0 12.5 12.5 17 25.0 37.5 12.5 25.0 18 0.0 62.5 25.0 12.5 19 0.0 37.5 25.0 37.5 20 12.5 25.0 37.5 25.0 21 25.0 62.5 12.5 0.0 22 12.5 12.5 75.0 0.0 23 12.5 12.5 12.5 62.5 24 12.5 12.5 37.5 37.5 25 0.0 50.0 12.5 37.5 26 0.0 12.5 75.0 12.5 27 25.0 25.0 12.5 37.5 28 37.5 37.5 0.0 25.0 29 25.0 25.0 50.0 0.0 30 12.5 0.0 75.0 12.5 31 25.0 50.0 12.5 12.5 32 12.5 37.5 25.0 25.0 33 25.0 25.0 37.5 12.5 34 25.0 12.5 62.5 0.0 35 0.0 25.0 12.5 62.5 36 37.5 25.0 12.5 25.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 1.5 33 3.0 34 3.0 35 1.5 36 0.0 37 0.0 38 0.0 39 0.0 40 1.0 41 2.0 42 2.0 43 2.0 44 2.0 45 2.0 46 1.0 47 0.0 48 0.0 49 0.5 50 1.0 51 0.5 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 8.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.5 #Duplication Level Percentage of deduplicated Percentage of total 1 85.71428571428571 75.0 2 14.285714285714285 25.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGGAAGATATTGGTAAATCATCTATCGTTGTTTCA 2 25.0 No Hit TTCAATATGTCCCTCAAAAGATCTCTGATTAAAGAC 1 12.5 No Hit CATCAACGCTATCACTTTCTGTTCACAAAGTATGCG 1 12.5 No Hit TCCTTGAGGTTAATTGCTCAGGCCCTTCGTAGGTCT 1 12.5 No Hit TAGTAAACTGAGGAGCGCATAACCTAAGTCCCATAG 1 12.5 No Hit GGGAATCGATGATAGAGACTTTGACTGAATACGACG 1 12.5 No Hit AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC 1 12.5 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE