FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_29.35200000038da4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_29.35200000038da4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669840
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT800.001659958613.1218428
GTATTAG4400.012.2712451
CTAGGAC1006.466351E-411.9967543
ACTGTCC2002.2349923E-811.2472938
TAGACAG2301.3187673E-911.0839575
TATTAGG4950.010.9067932
ACTGCGC1100.001436152510.9064668
TACACCG1807.905546E-710.8304025
AGGACAG1951.9036088E-710.7663165
GTCTTAG2651.09139364E-1010.7534181
ACAGAGC2104.5836714E-810.7117088
TAGGACC1551.961209E-510.6422824
AGATCGC1150.0020802710.4319596
CAGTGCA1756.6333323E-610.2832399
TGGACTG1756.6357024E-610.2829315
AACCGTC1356.9759676E-49.9972957
TAGACTG1653.9065453E-59.9972955
GTAGACT1653.9065453E-59.9972954
CTAATAC4250.09.879683
ACAGTGC2157.2689E-79.7650928
Produced by FastQC (version 0.11.2)