FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_28.35200000038d60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_28.35200000038d60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419964
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23660.16662394257882593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG1354.2089596E-612.22242451
GTATTAG3500.012.0001961
TAGGACT1006.453545E-411.99935154
GTACTAG2103.7380232E-911.428761
CAGGGCC950.00600019311.0520349
GGTAGTC1501.3606688E-510.9994067
TAGAGTG2053.205423E-810.9750185
TCTACAC2905.456968E-1210.8614833
TAGGTAG1951.8970422E-710.7686495
TCTGTAC1951.8970422E-710.7686493
TACTATG4700.010.5313467
TTAAGAC3009.094947E-1210.4994333
GTCTAGG1150.002074983410.4349541
GTATTAA3900.010.3847871
ACTATGC4200.010.3565848
CCCTTAT2352.1631422E-810.21293451
GTATAGA4450.010.1125261
CTAGACA1200.00295591269.999464
ACCGCTA1200.00295591269.999468
GGACTCT1356.960733E-49.999466
Produced by FastQC (version 0.11.2)