FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_27.35200000038d2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_27.35200000038d2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783397
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25080.1406304933786476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAG851.636619E-414.11523155
CTGATCG954.1925022E-412.6304829
CTAATAC4450.012.4697773
GTACTAG2600.012.1146721
CCTAGCG1006.44004E-412.00265930
TTAGGAC1755.490274E-711.1409513
GTATTAG4350.011.0338331
TAGGACA2053.2132448E-810.9737334
CAGCGCA1653.2772514E-610.9081439
CCCTAAG1000.00878410210.4993811
GTCTAGG1000.00878410210.4993811
GTTCAGG2156.457776E-810.46451
TGGACTC1602.7814544E-510.3107375
GTATTAT8450.010.1177471
ACCTAGC1356.944984E-410.00221629
CGGTGTT3451.8189894E-129.999139
GGACAGT2701.7280399E-99.9988516
CTATACT4850.09.8952144
TACGGTG3950.09.872567
CTAGAAC2301.7329148E-79.7809353
Produced by FastQC (version 0.11.2)