FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_26.35200000038cf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_26.35200000038cf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212738
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18780.15485620142190645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG1600.016.8741879
CCGCGGC600.003750132215.00237327
GCCGGAC600.0037542514.99989811
CCCCAGA1054.270525E-614.2856161
AGTCGGA750.001016469314.00279130
ACCGTGC750.001019007413.9981738
GGTAGTC2150.013.9516667
TAGGTAG2401.8189894E-1212.4983685
GTCCTAG850.002612539312.3528571
TAGACGG1354.2144475E-612.2206275
GAGTGCA1006.4497924E-411.99991711
TTAGAAC2401.8189894E-1111.873453
ACAGGGC1151.5804562E-411.7375978
CGATCAC900.004003803711.66803124
CCTAATA4500.011.6661062
ACCGCAC900.00401121211.6651448
ACTATGC4550.011.5369568
ATCACAC3000.011.50181929
CTATGCA3400.011.4700369
TCTAGCC1059.72209E-411.4270793
Produced by FastQC (version 0.11.2)