FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_25.35200000038cb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_25.35200000038cb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583371
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12800.21941440352708655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAG400.006509102418.74882110
CTAATAC1600.018.7472153
CGATTAG500.001109800417.99886914
ACCTAAT1600.017.8098551
TGCCTCG703.1075662E-517.1432518
CCAGGAG703.112143E-517.140313
CCTAATA1850.015.4031152
CGTAGTA901.5798394E-514.99905610
GTATTAT2600.014.9977721
TGTAACG600.003754678214.9977712
CTTAAGG952.6344382E-514.2084153
CTATGCA1701.3096724E-1014.1167599
TAATACT2550.014.115554
CTCTTAG851.633329E-414.1155491
ACTCCGC750.001016055814.0015226
GTATTAG2101.8189894E-1213.5694121
CTCCGCA902.6440865E-413.33592427
GGTAGTC800.001655488713.1241757
TGGACCC800.001656557713.123055
AGGTAGC954.184146E-412.6307857
Produced by FastQC (version 0.11.2)