FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_24.35200000038c7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_24.35200000038c7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629071
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14040.22318625401584238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAC455.468344E-419.9969796
CCCTACC559.5816635E-519.0880262
TAGTCAG559.5816635E-519.0880269
GGGTGCT703.112835E-517.1402687
ATTAGGC550.00210234116.3611663
TGGACTG550.00210234116.3611665
GGCCGAG901.5803591E-514.99892611
GTGTGAG600.003755175514.9977357
TAACCTA600.003755175514.9977355
GTCTAAC952.6331723E-514.2095091
TAGGTAG851.633684E-414.1155155
GAGCCGG650.006381476313.84626321
GCGCGTT650.006384838413.84516211
TGGGTGC650.006388201413.8440626
GACCAGC650.006388201413.8440626
ACCAGCG650.006388201413.8440627
TATTAGG1106.9082653E-613.6343042
AGAGTGT1004.2705477E-513.4979616
ATAACAC1251.7757811E-613.1980073
GAGTGTG1056.749863E-512.8552017
Produced by FastQC (version 0.11.2)